Improved protease
substrates
Ultra-high-specificity enzyme substrates:
Artificial enzyme substrates (protease and kinase substrate peptides, etc.)
have been the key to many advances in modern biology and biotechnology.
Yet, useful as they are, many of these substrates have sub optimal
specificity. There is too much cross-reaction with non-target enzymes.
This makes the analysis of complex biochemical pathways difficult. It
makes the creation of proteomic microarrays for complex enzymatic pathways
nearly impossible.Molecular Pathways has devised
generalized methods for rapidly developing new, ultra-high-specificity,
enzyme substrates (patent
pending) (text). These ultra-high-specificity substrates may be
used for improved proteomic microarrays, traditional microwell assays, or
any application where improved specificity is desired.
A PowerPoint
presentation with animated graphics, illustrating the concept, can be
downloaded
here.
Diagram showing a protease substrate fitting
into the active site of a protease enzyme.
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